Description
Quantity: 1 kit
Unit Size: Each
Kit leverages copper-catalyzed click chemistry and linoleamide alkyne (LAA) reagent (alkyne-modified linoleic acid) to detect lipid peroxidation-derived protein modifications in fixed cells. Linoleic acid is most abundant polyunsaturated fatty acid found in mammals and its lipid peroxidation products likely account for majority of lipid-derived protein carbonyls.
• Optimized for detection of lipid peroxidation in cells using click chemistry and Alexa Fluor™ 488 azide
• Multiplexible and compatible with traditional fluorescence microscopy, high content screening (HCS), and flow cytometry
• Provided as a complete kit sufficient for five 96-well plates or 100 coverslips
• Form: Frozen
• Excitation Class: VisibleHow It Works
• Click-iT Lipid Peroxidation Imaging Kit contains cumene hydroperoxide as ositive control for induction of lipid peroxidation and all of components for click reaction, including LAA, and Alexa Fluor™ 488 azide
• LAA can be incubated with cells, where it incorporates into cellular membranes
• Upon lipid peroxidation, LAA is oxidized and produces 9- and 13- hydroperoxy-octadecadienoic acid (HPODE)
• Hydroperoxides decompose to multiple α,β-unsaturated aldehydes, which readily modify proteins at nucleophilic side chains
• Alkyne-containing modified proteins can be subsequently detected using Click-iT chemistry and multiplexed with other probes appropriate for fixed cells
• Click-iT™ LAA (linoleamide alkyne) is stand-alone reagent provided as five pack set of single-use vials for maximum flexibility in assays for lipid peroxidation
• Reagent may be combined with variety of azide-modified detection reagents and other reagentsCell Analysis, Cell Health and Oxidative Stress with Click Chemistry, Cell Viability, Proliferation and Function, Cellular Imaging, Click Chemistries, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, High-Content Screening (HCS), Immunofluorescence (IF), Immunofluorescence Staining and Detection, Labeling Chemistry, Nitro-Oxidative Stress, Reactive Oxygen Species
