Thermo Fisher, C10644, Click-iT™ EdU Colorimetric IHC Detection Kit

Quantity: 50 Slides
Unit Size: Each
• Substrate: DAB
• Substrate Type: HRP
• The Click-iT™ EdU Colorimetric IHC (Immunohistochemistry) Detection Kit is a superior alternative to traditional methods for the colorimetric detection of proliferating cells within tissue sections. In this assay, 5-ethynyl-2'-deoxyuridine (EdU), a nucleoside analog of thymidine containing an alkyne moiety, is incorporated into newly synthesized DNA. When biotin containing an azide group is added, a brief click reaction attaches the azide to the alkyne moiety on the EdU molecule. Upon addition of streptavidin–peroxidase and a peroxidase substrate, an enzymatic reaction generates a signal that can be detected with light microscopy and stored for future analysis.
• Optimized - enables colorimetric detection of proliferating cells in either tissue or cell samples
• Efficient - no denaturation steps or harsh treatment required
• Robust - improved preservation of cell morphology and the cellular environment offers content-rich results
• Consistent - not dependent on variable antibody lots for detection
• Simple - designed to work consistently, in less time than traditional methods