Description
Quantity: 21 x 50 μL/tube
Unit Size: Each
One Shot INV110 Chemically Competent E. coli cells are derived from the JM110 strain and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. The dam and dcm deficiency allows for the production of plasmid DNA that is unmethylated at GmATC and CmC(A/T)GG sites. INV110 Chemically Competent cells can achieve a transformation efficiency of >1 x 10^6 cfu/μg of control DNA.The INV110 strain is a significant improvement over the JM110 strain. The INV110 strain has been engineered to carry an endA1 mutation, which eliminates the non-specific Endonuclease I, for improved plasmid DNA preparation. Mutation in the methylation-dependent restriction system Δ(mcrC-mrr) allows transformation of genomic DNA. This strain also has the lacZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. The INV110 strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids) and allows isolation of single-stranded DNA (ssDNA). In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG.One Shot INV110 Chemically Competent E. coli offer:• Transformation efficiencies of >1 x 10^6 cfu/μg• dam and dcm deficiency to allow restriction digestion with dam and dcm-sensitive restriction enzymes• Stable F´ episome that allows isolation of ssDNA and carries lacIq repressor for inducible expression from trc, tac, and lac promoters• Δ(mcrC-mrr) mutation that reduces cleavage of foreign methylated DNA and improves transformation of genomic DNA• lacZΔM15 for blue-white color screening of recombinant clones• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I• Tn10 to permit selection of the host strain using tetracyclineNote: dam deletion results in higher mutation rate, higher recombination frequency, and lower viability on plates. Avoid picking colonies that are noticeably larger than the rest of the population (potential revertants). Pick colonies and isolate the DNA within one day of transformation.Convenient and efficient formatOne Shot INV110 Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation, reducing both freeze-thaw cycles and costs associated with unused cells.GenotypeF´ [traD36 proAB lacIqlacZΔM15] rpsL (StrR) thr leu endA thi-1 lacY galK galT ara tonA tsx dam dcm supE44 Δ(lac-proAB) Δ(mcrC-mrr)102::Tn10(TetR)Find the strain and format that fits your needs • We offer other strains in chemically competent and electrocompetent cell formats to meet your specific needs.• Strains in MultiShot formats for high throughput applications are available.• Alternative strains for ssDNA production are available, such as TOP10F´ (Cat. No. C303003) or electrocompetent DH12S (Cat. No. 18312017).• Explore restriction enzyme details to find methylation sensitivity.
