| Certifications/Compliance | Conforms to CLSI standards. Industrial Reference: FDA. |
|---|---|
| Description | Mueller Hinton Agar |
| Quantity | 500 g |
| Yield | For 13.2L medium |
| Form | Powder |
| Product Type | Agar |
| Unit Size | Each |
Answer:The supplied unit contains 500 g of dehydrated medium, sufficient for approximately 13 L after preparation.
Answer:The published formulation uses 38 g of dehydrated powder per liter of distilled or purified water, followed by the specified sterilization procedure.
Answer:No. The un-supplemented medium is used mainly for non-fastidious organisms; fastidious species may require supplemented media and specific test conditions.
Thermo Scientific Oxoid Mueller Hinton Agar is a dehydrated microbiological medium used to prepare standardized agar plates for antimicrobial susceptibility testing. The 500 g powder format supports preparation of approximately 13 L of medium when reconstituted at the specified working concentration. The formulation combines beef infusion, casein hydrolysate, soluble starch and agar to support bacterial growth and diffusion of antimicrobial agents. Prepare the medium using the validated laboratory procedure, typically suspending 38 g per liter of purified water, dissolving completely and sterilizing before pouring plates at the required depth. Disc-diffusion assays should use the appropriate organism inoculum, incubation conditions, quality-control strains and interpretive standards. Un-supplemented Mueller Hinton Agar is primarily used for non-fastidious bacteria; fastidious organisms may require a different or supplemented medium. Consistent plate depth, pH and medium performance are important for reproducible inhibition-zone measurements.
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