Thermo Fisher, CM0617B, Bacillus cereus Selective Agar Base (Dehydrated)

Name: Bacillus cereus Selective Agar Base (Dehydrated)
Brand: Thermo Fisher
SKU: CM0617B

Quantity: 500 g, Unit Size: Each, Selectively isolate and enumerate Bacillus cereus from food samples with Thermo Scientific™ Oxoid™ Bacillus cereus Selective Agar Base (Dehydrated). Bacillus cereus Selective Agar Base meets the requirements for a medium that is sufficiently selective to be able to detect small numbers of Bacillus cereus cells and spores in the presence of large numbers of other food contaminants. The medium is also sufficiently diagnostic that colonies of Bacillus cereus are re

Catalog Number: CM0617B

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Category

Selective & Differential Media

Pack Size500 g

TagsMicrobiological Media and Media Additives Dehydrated Microbiology Media

CollectionsThermo Fisher Microbiological Media & Media Additives Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 g
Unit Size: Each
Bacillus cereus is a Gram-positive, rod-shaped beta hemolytic bacterium commonly found in soil and food. The role of Bacillus cereus in food poisoning, particularly from the consumption of contaminated rice, is now well documented^1,2,3. The organism has also been implicated in eye infections^4, 5 and a wide range of other conditions, including abscess formation, meningitis, septicemia and wound infection.Bacillus cereus is recognized as a significant pathogen in post-operative and post-traumatic wounds of orthopedic patients^6. Amongst veterinarians, Bacillus cereus is a known cause of disease, especially mastitis, in ewes and heifers^7.
• Differential: differentiates cereus from other species ofBacillusbased on their resistance to polymyxin, lack of mannitol fermentation, and the presence of lecithinase.
• Selective: inhibits the growth of gram-negative bacteria such asEscherichia coliandPseudomonas aeruginosadue to the addition of polymixin B supplement.Bacillus cereus Selective Agar is based on the highly specific diagnostic and selective PEMBA medium, developed by Holbrook and Anderson^1 for the isolation and enumeration of Bacillus cereus in foods. It meets the requirements for a medium that is sufficiently selective to be able to detect small numbers of Bacillus cereus cells and spores in the presence of large numbers of other food contaminants. The medium is also sufficiently diagnostic that colonies of Bacillus cereus are readily identified and confirmed by microscopic examination.The primary diagnostic features of the medium are the colonial appearance, precipitation of hydrolyzed lecithin and the failure of Bacillus cereus to utilize mannitol. The typical colonies of Bacillus cereus are crenated, about 5mm in diameter and have a distinctive turquoise to peacock blue color surrounded by a good egg yolk precipitate of the same color. These features distinguish Bacillus cereus from other Bacillus spp. except Bacillus thuringiensis.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand

Specifications

General

Description	Bacillus Cereus Selective Agar Base
Quantity	500 g
Yield	For 12.2L medium
Form	Powder
Product Type	Agar
Unit Size	Each

FAQs

Question: What quantity is supplied?

Answer:This configuration contains 500 g of dehydrated agar base.

Question: How much prepared medium can be made?

Answer:The 500 g pack yields approximately 12.2 L.

Question: What organism is the medium designed to isolate?

Answer:It is designed for selective isolation and enumeration of Bacillus cereus.

Application Note

Bacillus cereus Selective Agar Base is a selective and differential dehydrated medium for isolation and enumeration of Bacillus cereus from food samples. This configuration contains 500 g and prepares approximately 12.2 L of finished medium. The formulation differentiates B. cereus through characteristic colony appearance, lecithinase activity, and failure to utilize mannitol. When used with the appropriate selective supplement, competing flora are suppressed and presumptive B. cereus colonies develop a distinctive turquoise to peacock-blue appearance with an egg-yolk precipitate. These features support rapid recognition before microscopic or other confirmatory testing. The medium is supplied as a dry base for laboratory preparation.

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