Thermo Fisher, CM0729B, Dichloran-Glycerol (DG18) Agar Base (Dehydrated)

Name: Dichloran-Glycerol (DG18) Agar Base (Dehydrated)
Brand: Thermo Fisher
SKU: CM0729B

Description: Dichloran-Glycerol (DG18) Agar Base, Unit Size: Each, Isolate and enumerate yeasts and molds associated with food spoilage with selective low water activity Thermo Scientific™ Oxoid™ Dichloran-Glycerol (DG18) Agar Base (Dehydrated). The medium contains Dichloran which inhibits spreading of mucoraceous fungi and restricts the colony size of the genera. This restrictive characteristic makes the medium especially suitable for enumeration because it allows the growth of background organ

Catalog Number: CM0729B

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Category

Selective & Differential Media

TagsMicrobiological Media and Media Additives Dehydrated Microbiology Media

CollectionsThermo Fisher Microbiological Media & Media Additives Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Description: Dichloran-Glycerol (DG18) Agar Base
Unit Size: Each
Dichloran-Glycerol (DG18) Agar Base is based on the formulation described by Hocking and Pitt^1 and is recommended for the enumeration and isolation of xenophilic molds from dried and semi-dried foods like dried fruits, species, confectionery, cereals, nuts, dried meat and fish products.
• Makes colony counting easier: contains antifungal agent Dichloran which inhibits spreading of mucoraceous fungi and restricts the colony size of other genera.
• Allows greater recovery of xenophilic molds due to its low water activity as compared to other high water activity media.The medium formulation contains glycerol at 16% (w/w) which lowers the water activity (aW) from 0.999 to 0.95. Glycerol was chosen because of advantages it showed over sodium chloride and sugars which have traditionally been used to formulate media of reduced aW2.In a comparative study carried out between Dichloran-Glycerol (DG18) Agar Base and Dichloran Rose Bengal Chloramphenicol Agar (DRBC), (a medium of higher aW) greater recovery of xerophilic molds was achieved on the DG18 medium^1. In this study, it was found that two of the fungi commonly isolated from dried foods in high numbers, Aspergillus penicilloides and Wallemia sebi, grow very poorly or not at all on DRBC.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications

General

Certifications/Compliance	Industrial Reference: NMKL standards.
Description	Dichloran-Glycerol (DG18) Agar Base
Quantity	500 g
Yield	For 15.9L medium
Form	Powder
Product Type	Agar
Unit Size	Each

FAQs

Question: What organisms is DG18 agar used to recover?

Answer:It is used to isolate and enumerate yeasts and molds, especially from lower-water-activity foods.

Question: How much prepared medium can 500 g produce?

Answer:The 500 g package yields approximately 15.9 L.

Question: Why is dichloran included?

Answer:It restricts fungal spreading and colony size, helping separate colonies for counting.

Application Note

Dichloran-Glycerol (DG18) Agar Base is a dehydrated selective medium for isolation and enumeration of yeasts and molds associated with food spoilage. The formulation uses reduced water activity to favor xerophilic fungi and contains dichloran to restrict spreading by mucoraceous fungi and limit colony size, improving separation and counting on crowded plates. This 500 g package yields approximately 15.9 L of prepared medium. DG18 is useful for foods and ingredients with lower water activity, where conventional fungal media may allow excessive colony spreading or provide less selective recovery. The powder format is prepared as culture medium before use and is suitable for laboratory microbiology workflows aligned with relevant industrial methods, including NMKL applications. Incubation conditions and interpretation should follow the validated food or environmental testing method in use.

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