Thermo Fisher, CM0990B, EC Broth with Reduced Bile Salts (Dehydrated)

Quantity: 500 g
Unit Size: Each
Enterohemorrhagic strains of Escherichia coli and the serotype O157:H7 have presented an increasing concern among food and clinical microbiologists since their role as a causative agent in two major food related outbreaks of hemorrhagic colitis was established in 1982. Although a variety of serotypes are known to produce verotoxins; a three-year study in England and Wales showed that 99% of isolates from patients suffering diarrhoea, bloody diarrhoea or hemolytic uraemic syndrome (HUS) belonged to serogroup O157.^3EC Broth with Reduced Bile Salts is recommended by USDA/FSIS^4 and AIFST^1 as enrichment medium for the detection of Escherichia coli O157:H7 and O157: NM (Non-Motile) from meat and poultry products when used with Novobiocin Supplement SR0181.When using the reduced temperature of 37°C suggested by Okrend, supplementation with Novobiocin Supplement SR0181 at a final concentration of 20mg per litre improves reduction in background flora with no reduction in growth of Escherichia coli O157.^5