Thermo Fisher, D399, H2DCFDA (H2-DCF, DCF)

Quantity: 100 mg
Unit Size: Each
The cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H_2DCFDA) (also known as dichlorofluores cin diacetate) detects reactive oxygen species (ROS) in cells, for example to detect generation of reactive oxygen intermediates in neutrophils and macrophages. Upon cleavage of the acetate groups by intracellular esterases and oxidation, nonfluorescent H_2DCFDA is converted to highly fluorescent 2',7'-dichlorofluorescein (DCF).
• Ex/Em: ∽492 - 495/517 - 527nm
• Product is air sensitive (store under dry argon or nitrogen)
• Product may be dissolved in DMSO, DMF, or ethanol for use
• Indicator is cell permeant
• Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluoresceinRefer to the Molecular Probes™ Handbook for additional product information.Apoptosis, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Free Radical Detection, Nitro-Oxidative Stress, Reactive Oxygen Species