Thermo Fisher, E6639, EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

Quantity: 1 Kit
Unit Size: Each
The EnzChek Protease Assay Kit, red fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with a fluorescence microplate reader, is proportional to protease activity. This EnzChek Protease Assay Kit contains a casein derivative that has been extensively labeled with the red-fluorescent BODIPY TR-X dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY TR-X dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The red-fluorescent BODIPY TR-X dye has excitation and emission spectra similar to those of the Texas Red fluorophore.
• Generalized mechanism allows for measurement of variety of proteases
• Can be used to continuously measure protease kinetics
• Excitation/emission similar to Texas Red™ dye (589/617nm)Enzyme and Protein Activity Assays, Protease/Peptidase Activity, Proteins, Expression, Isolation and Analysis