Thermo Fisher, EC6655BOX, Novex™ IEF Protein Gels, pH 3-10, 1.0 mm

Name: Novex™ IEF Protein Gels, pH 3-10, 1.0 mm
Brand: Thermo Fisher
SKU: EC6655BOX

Wells: 10-well, Unit Size: Each, Novex™ pH 3-10 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.

Catalog Number: EC6655BOX

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Category

Pre-Cast Polyacrylamide Gels

TagsGel Electrophoresis Equipment and Supplies Precast Electrophoresis Gels Isoelectric Focusing Gels and Strips

CollectionsThermo Fisher Gel Electrophoresis: Agarose Gels, SDS-PAGE & Blotting Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Wells: 10-well
Unit Size: Each
IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours.FormulationNovex™ IEF gels are 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes. They do not contain denaturing reagents.Recommended buffersIEF is a sensitive technique which is affected by many factors. Our optimized, premixed IEF buffers reduce variability and help you obtain consistent results.

Specifications

General

For Use With (Equipment)	XCell SureLock Mini-Cell
Gel Thickness	1.0 mm
Quantity	5 Gels/Box
Storage Requirements	Store at 2°C to 8°C. Do not freeze.
Gel Type	IEF
Wells	10-well
Unit Size	Each

FAQs

Question: What pH range is used?

Answer:The gels use a pH 3–10 isoelectric-focusing range.

Question: What is the gel format?

Answer:Each gel is 1.0 mm thick with 10 wells.

Question: How many gels are supplied?

Answer:The box contains five gels.

Application Note

Novex™ IEF Protein Gels are pre-cast polyacrylamide gels for isoelectric focusing of soluble proteins across a pH 3–10 gradient. This configuration uses a 1.0 mm gel thickness, a 10-well format, and is supplied as five gels per box. Isoelectric focusing separates proteins based on isoelectric point rather than molecular mass, causing each protein to focus where its net charge approaches zero. The gels are designed for use with the XCell SureLock™ Mini-Cell and can support pI determination or first-dimension separations in compatible protein-analysis workflows. The broad pH 3–10 range is useful for general screening when a narrower pH interval has not yet been selected. Store the gels at 2–8 °C and do not freeze.

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