Thermo Fisher, EP0452, RevertAid H Minus Reverse Transcriptase (200 U/μL), 5 x 10,000 Units

Quantity: 5 x 10,000 Units
Unit Size: Each
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C).Features of RevertAid H Minus Reverse Transcriptase include:• High yields of full-length first strand cDNA up to 13 kb• Optimum activity at 42–45°C• Active up to 55°C• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)Applications• First strand cDNA synthesis for RT-PCR and real-time RT-PCR• Reverse transcription at elevated temperatures to reduce effects of secondary structure• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension