Description
Color: Colorless
No. of Reactions: 1000 Reactions
Unit Size: Each
The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.Features
• No reactivation step with hot start
• Amplification four times faster than hot startTaq
• Minimal reaction optimization due to high inhibitor tolerance
• Abundant products due to high efficiency
• Amplifies significantly longer DNA fragments than hot startTaq
• Direct loading on gel with Green BufferApplications
• Hot Start PCR
• Standard PCR
• Non-high-fidelity PCR
• Fast PCR
• High throughput PCR
• GenotypingNotes
• The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that ofTaq-based polymerases.
