Thermo Fisher, F14221, Fluo-5F, Pentapotassium Salt, cell impermeant

Quantity: 500 μg
Unit Size: Each
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca^2+.
• Cells may be physically loaded with cell-impermeant salt forms of these indicators using patch pipet or microinjection or Influx™ pinocytotic cell-loading reagent
• Compatible with excitation at 488nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications
• Exhibit fluorescence increase upon binding Ca^2+with little shift in wavelength
• BAPTA-based indicators bind various heavy metal cations (e.g., Mn^2+, Zn^2+, Pb^2+) with substantially higher affinity than Ca^2+
• Perturbations to calcium measurements caused by presence of the ions can be controlled using heavy metal-selective chelator
• Label (Ex/Em of Ca^2+-bound form): Fluo-5N (494/516nm)
• Fluorescence intensity increase upon binding Ca^2+: >100 fold
• K_dfor Ca^2+in buffer: approximately 90μMCalcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling