Thermo Fisher, F175L, Phusion Blood Direct PCR Master Mix

Name: Phusion Blood Direct PCR Master Mix
Brand: Thermo Fisher
SKU: F175L

No. of Reactions: 500 Reactions, Unit Size: Each, Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

Catalog Number: F175L

Pricing

Send an RFQ.

View RFQ List

Category

PCR Master Mixes

Pack Size175 L

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Direct PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 500 Reactions
Unit Size: Each
This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions, and from various species.Highlights
• Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
• Available Dilution & Storage protocol allows several PCR reactions from one sample
• Specially engineered Phire Hot Start II DNA polymerase for extremely short PCR protocol times
• Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gelApplications
• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing
• CloningNotes
• Annealing temperature for Phire DNA Polymerases may differ significantly from that ofTaq-based polymerases.

Specifications

General

Hot Start	Built-In Hot Start
Includes Label or Dye	Yes
No. of Reactions	500 Reactions
Overhang	Blunt
Polymerase	Phusion Blood II DNA Polymerase
Product Type	Direct PCR Master Mix
Quantity	500 reactions
Reaction Format	SuperMix or Master Mix
Sample Type	Blood
Shipping Condition	Wet or Dry Ice
Starting Material	DNA
Concentration	2X
For Use With (Application)	Genotyping
GC-Rich PCR Performance	High
PCR Method	Direct PCR
Reaction Speed	Fast
Unit Size	Each

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.

Thermo Fisher, F175L, Phusion Blood Direct PCR Master Mix

Send an RFQ.

General

Notice

More from Thermo Fisher.

NaCl (5 M), RNase-free, 100 mL

Potassium Acetate (3 M), pH 5.5, RNase-free

SDS, 20% Solution, RNase-free

RNase A, DNase and protease-free (10 mg/mL)

More from Thermo Fisher.

NaCl (5 M), RNase-free, 100 mL

Potassium Acetate (3 M), pH 5.5, RNase-free

SDS, 20% Solution, RNase-free

RNase A, DNase and protease-free (10 mg/mL)