Thermo Fisher, F23980, Fluo-4FF, Pentapotassium Salt, cell impermeant

Quantity: 500 μg
Unit Size: Each
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca^2+. Fluo-5F, fluo-5N, and fluo-4ff are analogs of fluo-4 with lower Ca^2+-binding affinity, making them suitable for detecting intracellular calcium levels in the 1μM to 1 mM range that would saturate the response of fluo-3 and fluo-4. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection or our Influx™ pinocytotic cell-loading reagent. These indicators are compatible with excitation at 488 nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications.Calcium Indicator (Cell-Impermeant Salts) Specifications:
• Label (Ex/Em of Ca^2+–bound form): Fluo-4FF (494/516 nm)
• Fluorescence intensity increase upon binding Ca^2+: >100 fold
• K_dfor Ca^2+in buffer: ∼9.7μM
• Exhibit fluorescence increase upon binding Ca^2+with little shift in wavelengthUsing TPEN to Control Heavy Metal CationsIn addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn^2+, Zn^2+, Pb^2+) with substantially higher affinity than Ca^2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.More Choices for Fluorescent Calcium IndicatorsWe offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, review Fluorescent Ca^2+Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes™ Handbook.For UV-excitable Ca^2+indicators, protein-based Ca^2+indicators, conjugates of Ca^2+indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg^2+, Zn^2+) review Indicators for Ca^2+, Mg^2+, Zn^2+and Other Metal Ions—Chapter 19 in the Molecular Probes™ Handbook.For Research Use Only. Not for human or animal therapeutic or diagnostic use.