Thermo Fisher, F521L, Phusion™ HF and GC Buffer Packs

Name: Phusion™ HF and GC Buffer Packs
Brand: Thermo Fisher
SKU: F521L

Chemical Name or Material: Detergent-free GC Buffer Pack, Color: Colorless, Unit Size: Each, The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications

Catalog Number: F521L

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Category

PCR Buffers

Pack Size521 L

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products PCR Specific Buffers

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Chemical Name or Material: Detergent-free GC Buffer Pack
Color: Colorless
Unit Size: Each
5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:
• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Specifications

General

Chemical Name or Material	Detergent-free GC Buffer Pack
Color	Colorless
Description	Detergent-free Phusion™ GC Buffer Pack
Includes	• 5X Phusion GC Reaction Buffer, Detergent-free (4 x 1.5 mL)<br />• 50 mM MgCl<sub>2</sub> (4 x 1.5 mL)<br />• DMSO (0.5 mL)<br /><br />Store at –20°C.
For Use With (Application)	Standard PCR
Quantity	4 x 1.5 mL
Unit Size	Each

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Thermo Fisher, F521L, Phusion™ HF and GC Buffer Packs

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