Thermo Fisher, F527L, Phire Reaction Buffers

Name: Phire Reaction Buffers
Brand: Thermo Fisher
SKU: F527L

Chemical Name or Material: Phire Green Reaction Buffer, Unit Size: Each, Thermo Scientific Phire Reaction Buffer is designed for Phire Hot Start II DNA Polymerase. This 5X buffer solution is available in three formats, a non-colored version with or without detergents and a green version that contains a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such a

Catalog Number: F527L

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Category

PCR Buffers

Pack Size527 L

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products PCR Specific Buffers

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Chemical Name or Material: Phire Green Reaction Buffer
Unit Size: Each
Phire Reaction Green Buffer includes a blue die that migrates with 3–5 kb DNA fragments in 1% agarose gel and a yellow dye that migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm.The concentration of Mg^2+ in Phire Reaction Buffer is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl_2 at final reaction concentration. Separate tubes of MgCl_2 and DMSO are provided for further optimization.Notes
• For applications that require PCR product analysis by absorbance or fluorescence excitation, the colorless Phire Reaction Buffer is recommended.

Specifications

General

Chemical Name or Material	Phire Green Reaction Buffer
Description	Green
Includes	• 5X Phire Green Reaction Buffer, 4 x 1.5 mL<br />• 50 mM MgCl<sub>2</sub>, 2 x 1.5 mL<br />• DMSO, 0.5 mL<br /><br />Store at -20°C.
Concentration	5X
For Use With (Application)	Hot-start PCR
Quantity	4 x 1.5 mL
Unit Size	Each

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Thermo Fisher, F527L, Phire Reaction Buffers

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