Thermo Fisher, F530N, Phusion™ High-Fidelity DNA Polymerase & dNTP Mix (10 mM each)

Name: Phusion™ High-Fidelity DNA Polymerase & dNTP Mix (10 mM each)
Brand: Thermo Fisher
SKU: F530N

Quantity: 2000 reactions, Unit Size: Each, Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR. Featuring an error rate 52-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors and generate higher yields with l

Catalog Number: F530N

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Category

DNA Polymerases

Pack Size2000 reactions

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products High Fidelity PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 2000 reactions
Unit Size: Each
Features
• High fidelity (52XTaq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amountsApplications
• Standard PCR
• RT-PCR
• High fidelity and long PCR
• LAMP, RDA, and MDA
• cDNA synthesis
• qPCR
• DNA labeling and sequencingdNTP MixThermo Scientific dNTP Mix contains pre-mixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity and are free of nuclease activities, as well as human and E. coli DNA. Mixes offer the possibility of reducing the number of pipetting steps and the risk of reaction setup errors.dNTP features
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C, 3 minutes at 72°C)Notes
• Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such asTaqDNA polymerases).

Specifications

General

Fidelity (vs. Taq)	52X
Hot Start	No
No. of Reactions	2000 Reactions
Overhang	Blunt
Polymerase	Phusion High Fidelity DNA Polymerase
Product Type	High-Fidelity DNA Polymerase
Quantity	2000 reactions
Reaction Format	Separate Components
Shipping Condition	Dry Ice
Size (Final Product)	20 kb or less
Concentration	2 U/μL
Detection Method	Primer-probe
For Use With (Application)	Standard PCR
GC-Rich PCR Performance	High
PCR Method	1-step RT-qPCR
Reaction Speed	Fast
Unit Size	Each

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Thermo Fisher, F530N, Phusion™ High-Fidelity DNA Polymerase & dNTP Mix (10 mM each)

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General

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