Thermo Fisher, F541, Phusion Site-Directed Mutagenesis Kit

Name: Phusion Site-Directed Mutagenesis Kit
Brand: Thermo Fisher
SKU: F541

Quantity: 20 reactions, Unit Size: Each, Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into an

Catalog Number: F541

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Category

Sequencing Reaction Kits

Pack Size20 reactions

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products High Fidelity PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 20 reactions
Unit Size: Each
Highlights
• Robust and reliable exponential amplification method
• No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
• No need to destroy the starting template in a separate step
• Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations
• Amplification of large plasmids up to 10 kb
• Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
• T4 DNA Ligase included in the kit; no purification steps before or after ligation
• Compatible with all strains of competentE. colicells

Specifications

General

Fidelity (vs. Taq)	52X
Format	Kit
Hot Start	Yes
Mutagenesis Capability	Single-Site Mutagenesis
No. of Reactions	20 Reactions
Polymerase	Phusion Hot Start II High Fidelity DNA Polymerase
Product Line	Phusion
Product Type	Site-Directed Mutagenesis Kit
Quantity	20 reactions
Starting Material	DNA
Form	Solution
GC-Rich PCR Performance	High
Reaction Speed	Fast
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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Thermo Fisher, F541, Phusion Site-Directed Mutagenesis Kit

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General

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