Thermo Fisher, F548S, Phusion Flash High-Fidelity PCR Master Mix

Name: Phusion Flash High-Fidelity PCR Master Mix
Brand: Thermo Fisher
SKU: F548S

No. of Reactions: 100 Reactions, Unit Size: Each, Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols. The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less). The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DN

Catalog Number: F548S

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Category

PCR Master Mixes

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products High Fidelity PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 100 Reactions
Unit Size: Each
Features
• Extreme speed with extension times of 15 s/kb or less
• Hot start modification allows 'zero-time reactivation'
• Accurate proofreading DNA polymerase with a fidelity of 25XTaq
• High yields in reduced cycling time
• Minimized pipetting stepsApplications
• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing Multiplex PCR
• Long-range PCR
• Cloning & mutagenesis
• MicroarrayNotes
• Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such asTaqDNA polymerases).

Specifications

General

Color	Colorless
Fidelity (vs. Taq)	25X
Hot Start	Built-In Hot Start
No. of Reactions	100 Reactions
Overhang	Blunt
Polymerase	Phusion Flash II DNA Polymerase
Product Type	High-Fidelity PCR Master Mix
Quantity	100 reactions
Reaction Format	SuperMix or Master Mix
Shipping Condition	Dry Ice
Size (Final Product)	20 kb or less
Concentration	2X
For Use With (Application)	Hot-start PCR
GC-Rich PCR Performance	High
Reaction Speed	Fast
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
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Thermo Fisher, F548S, Phusion Flash High-Fidelity PCR Master Mix

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General

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