Thermo Fisher, F553L, Phusion High-Fidelity PCR Kit

Name: Phusion High-Fidelity PCR Kit
Brand: Thermo Fisher
SKU: F553L

No. of Reactions: 200 Reactions, Unit Size: Each, Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yi

Catalog Number: F553L

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Category

DNA Polymerases

Pack Size553 L

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products High Fidelity PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 200 Reactions
Unit Size: Each
The Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 μL volume or 50 control reactions in 20 μL volume.Highlights
• High fidelity (52XTaq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• Increased product yields with minimal enzyme amountsApplications
• High-fidelity PCR
• High throughput
• Amplification of difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning & mutagenesis
• MicroarrayNotes
• Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such asTaqDNA polymerases).

Specifications

General

Fidelity (vs. Taq)	52X
Hot Start	No
No. of Reactions	200 Reactions
Overhang	Blunt
Polymerase	Phusion High-Fidelity DNA Polymerase
Product Type	High-Fidelity PCR Kit
Quantity	200 reactions
Reaction Format	Separate Components
Shipping Condition	Dry Ice
Size (Final Product)	20 kb or less
For Use With (Application)	High-fidelity PCR
GC-Rich PCR Performance	High
Reaction Speed	Fast
Unit Size	Each

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Thermo Fisher, F553L, Phusion High-Fidelity PCR Kit

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General

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