Thermo Fisher, F555S, Phusion U Hot Start DNA Polymerases

Name: Phusion U Hot Start DNA Polymerases
Brand: Thermo Fisher
SKU: F555S

Color: Colorless, No. of Reactions: 100 Reactions, Unit Size: Each, Thermo Scientific Phusion U DNA Polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion polymerase, Phusion U polymerase overcomes an important limitation of proofreading enzymes: it is able to incorporate dUTP and read through uracil present in DNA templates.

Catalog Number: F555S

Pricing

Send an RFQ.

View RFQ List

Category

High-Fidelity Polymerases

Pack Size100 units / pack

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products High Fidelity PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Color: Colorless
No. of Reactions: 100 Reactions
Unit Size: Each
In addition to the uracil usage possibility, Phusion U polymerase features all the superior properties of other Phusion DNA polymerases: great accuracy, speed, ability to amplify long amplicons up to 20 kb, and a high specificity with Affibody-based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.Highlights
• Accuracy—high fidelity DNA polymerase (25X Taq)
• Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates
• Specificity—hot start for reduced nonspecific amplification and primer degradation
• Speed—short extension times (15–30 s/kb)
• Green format—permits direct loading of PCR products on gelsApplications
• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methodsUsing Phusion DNA polymerasesAnnealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

General

Color	Colorless
Fidelity (vs. Taq)	25X
Hot Start	Built-In Hot Start
No. of Reactions	100 Reactions
Overhang	Blunt
Polymerase	Phusion U Hot Start DNA Polymerase
Product Type	Hot Start DNA Polymerase
Quantity	100 units
Reaction Format	Standalone
Shipping Condition	Dry Ice
Size (Final Product)	20 kb or less
Concentration	2 U/μL
For Use With (Application)	High-fidelity PCR
GC-Rich PCR Performance	High
Reaction Speed	Fast
Unit Size	Each

FAQs

Question: What is distinctive about Phusion U Hot Start DNA Polymerase?

Answer:It can incorporate dUTP and amplify uracil-containing DNA while retaining high-fidelity proofreading activity.

Question: How much enzyme is supplied?

Answer:This configuration contains 100 units at 2 U/µL.

Question: What type of PCR products are generated?

Answer:Phusion U generates blunt-ended PCR products.

Application Note

Phusion U Hot Start DNA Polymerase is used for high-fidelity PCR when templates contain uracil or workflows require dUTP incorporation. The engineered polymerase includes a mutation in the dUTP-binding pocket that allows amplification of uracil-containing DNA, overcoming a common limitation of proofreading polymerases. The enzyme provides approximately 25-fold higher fidelity than Taq, built-in Affibody-based hot start, fast extension and amplification of products up to approximately 20 kb. It is particularly useful for bisulfite-converted DNA, damaged or aged DNA, carryover-contamination control and USER cloning workflows. This colorless configuration contains 100 units at 2 U/µL for approximately 100 reactions and is supplied with Phusion HF and GC buffers, DMSO and MgCl2 solution. PCR products are blunt ended.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.