Description
Quantity: 192 Tests/Kit
Unit Size: Each
• THC (a member of the cannabinoid family) is the primary psychoactive ingredient of marijuana (1).
• Cannabinoid metabolites appear in urine two to four hours after a marijuana smoke and may persist for days (up to thirty) (1- 3).
• Thus a urine assay reasonably serves to detect cannabis use even though a considerable period may have elapsed since smoking or ingestion of marijuana. The Cannabinoids Direct ELISA Kit is based upon the competitive binding to antibody of enzyme labeled antigen and unlabeled antigen, in proportion to their concentration in the reaction mixture. A 10 μL aliquot of a diluted unknown specimen is incubated with a 100 μL dilution of enzyme (Horseradish peroxidase) labeled carboxy THC (THCA) derivative in micro-plate wells, coated with fixed amounts of oriented high affinity purified polyclonal antibody. The wells are washed thoroughly and a chromogenic substrate added. The color produced is stopped using a dilute acid stop solution and the wells read at 450 nm. The intensity of the color developed is inversely proportional to the concentration of drug in the sample. The technique is sensitive to 1 ng/mL. The THC Direct ELISA Kit avoids extraction of urine or blood sample for measurement. It employs a polyclonal high affinity, purified carboxy THC antibody. Due to the proprietary method of orienting the antibody on the polystyrene micro-plate much higher sensitivity is achieved compared to passive adsorption. This results in extremely small sample size reducing matrix effects and interference with binding proteins(s) or other macromolecules.
