Thermo Fisher, J63615.AC, Laemmli SDS sample buffer, non-reducing (4X)

Name: Laemmli SDS sample buffer, non-reducing (4X)
Brand: Thermo Fisher
SKU: J63615.AC

Quantity: 25 mL, Unit Size: Each, Laemmli SDS sample buffer is used for protein sample preparation to be used in the Laemmli SDS-PAGE system, The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. This Thermo Scientific Chemicals brand product was originally part of the Alf

Catalog Number: J63615.AC

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Category

Electrophoresis Power Supplies

Pack Size25 mL

TagsGel Electrophoresis Equipment and Supplies Gel Electrophoresis Reagents and Buffers Gel Electrophoresis Buffers and Diluents

CollectionsThermo Fisher Gel Electrophoresis: Agarose Gels, SDS-PAGE & Blotting Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 25 mL
Unit Size: Each
General Description
• Laemmli SDS sample buffer is composed of several compounds, including 375 mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 9% β-mercaptoethanol, 0.03% bromophenol blue
• The SDS compound present in the Laemmli SDS sample buffer binds noncovalently to proteins. SDS is negatively charged and can mask the intrinsic charge of the protein it bindsApplication
• Laemmli SDS sample buffer is used to denature proteins and for protein sample preparation to be used in the SDS-PAGE system
• This sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of the running gel
• It is also used for polyacrylamide protein gel analysis

Specifications

General

Concentration	4X
For Use With (Application)	Laemmli SDS-PAGE
Form	Liquid
Quantity	25 mL
Color	Blue
Product Type	Sample Loading Buffer
Unit Size	Each

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Thermo Fisher, J63615.AC, Laemmli SDS sample buffer, non-reducing (4X)

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