Thermo Fisher, J64000.MA, Doxorubicin hydrochloride

Quantity: 10 mg
Unit Size: Each
General Description
• Doxorubicin hydrochloride, isolated from the bacterium Streptomyces peucetius, is an anthracycline antibiotic with antineoplastic activity and is related to daunorubicin
• It can intercalate between base pairs in the DNA helix preventing DNA replication
• It also inhibits the nucleic acid synthesis and can induce apoptosis
• It can inhibit DNA topoisomerase II, resulting in an increased and stabilized cleavable enzyme-DNA linked complex during DNA replication, and prevents nucleotide strand ligation after double-strand breakage
• Doxorubicin hydrochloride forms oxygen free radicals that result in cytotoxicity and lead to the lipid peroxidation of cell membrane lipidsApplication
• This compound displays a fluorescent property allowing the measurement of drug efflux pump activity and examining the role of subcellular organelles (Golgi complex and lysosome) in the sequestration of drugs
• It has been used in cell viability assays
• It is used as a drug in polymeric PLGA-based microparticulate drug delivery and to develop in vitro doxorubicin-resistant HepG2 cells (HepG2-DR) and K562 cells (K562-DR)
• It is a substrate of the MRP1 transporter