Thermo Fisher, K0172, PCR Master Mix (2X)

Name: PCR Master Mix (2X)
Brand: Thermo Fisher
SKU: K0172

No. of Reactions: 1000 Reactions, Unit Size: Each, Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Catalog Number: K0172

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Category

PCR Master Mixes

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Standard PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 1000 Reactions
Unit Size: Each
Highlights
• Convenient, ready-to-use mix
• Thermostable—half life is more than 40 min. at 95°C
• Generates PCR products with 3'-dA overhangs
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labelingNoteThe error rate of Taq DNA Polymerase in PCR is 2.2 x 10^-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 10^4. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.

Specifications

General

Fidelity (vs. Taq)	1X
Hot Start	No
No. of Reactions	1000 Reactions
Overhang	3'-A
Polymerase	<i>Taq</i> DNA Polymerase
Product Type	PCR Master Mix
Quantity	1,000 reactions
Reaction Format	SuperMix or Master Mix
Shipping Condition	Dry Ice
Starting Material	DNA
Concentration	2X
For Use With (Application)	Standard PCR
GC-Rich PCR Performance	Low
Reaction Speed	Standard
Unit Size	Each

Ordering

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Thermo Fisher, K0172, PCR Master Mix (2X)

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General

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