Thermo Fisher, K0771, Fast DNA End Repair Kit

Name: Fast DNA End Repair Kit
Brand: Thermo Fisher
SKU: K0771

Quantity: 50 Reactions, Unit Size: Each, Fast DNA End Repair Kit is used for blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation.All components of the kit contain premixed reagents to reduce pipetting steps and provide convenience. The End Repair Enzyme Mix contains an optimized mixture of T4 DNA Polymerase and Klenow Fragment to achieve highly effective blunting of fragmented DNA, and T4 Polynucleotide Kinase (PKN) for efficient phosphorylation of

Catalog Number: K0771

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Category

Restriction Enzymes

Pack Size50 reactions

TagsBiochemical Reagents DNA Reagents DNA Cloning and Mapping and Mutagenesis

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 50 Reactions
Unit Size: Each
HighlightsDuring the DNA end repair reaction, fragmented DNA is converted into blunt-end DNA containing a 5'-phosphate and 3'-hydroxyl groups. The 5'→3' polymerase activity of the End Repair Enzyme Mix fills-in 5' protruded DNA ends while 3'→5' exonuclease activity removes 3'-overhangs. T4 PNK adds 5'-phosphates to ends of unphosphorylated DNA fragments, such as PCR products.Highlights
• Efficient- blunting and phosphorylation of 0.5 to 5 µg DNA
• Fast-reaction is completed in 5 minutes
• Convenient-reaction components are premixed to reduce pipetting steps

Specifications

General

Enzyme	DNA Polymerase, T4 Polynucleotide Kinase
For Use With (Application)	Cloning
Product Type	Fast DNA End Repair Kit
Quantity	50 Reactions
Sample Type	DNA
Format	Kit
Unit Size	Each

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Thermo Fisher, K0771, Fast DNA End Repair Kit

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General

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