Thermo Fisher, K145501, β-Gal Assay Kit

Quantity: 80 mL
Unit Size: Each
• lacZis bacterial gene often used as reporter construct in eukaryotic transfection experiments
• Gene product oflacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
• β-galactosidase catalyzes hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG)
• Hydrolysis of ONPG to ONP anion produces bright yellow color with peak absorbance at 420nm that can be quantified using spectrophotometer
• 10.7 gene expression
• Colorimetric detectionBeta-Galactosidase Assays, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays