Thermo Fisher, K1632, RevertAid H Minus First Strand cDNA Synthesis Kit, 100 reactions

No. of Reactions: 100 Reactions
Unit Size: Each
Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.Features of the RevertAid H Minus First Strand cDNA Synthesis Kit include:• High yields of full-length first-strand cDNA up to 13 kb• Increased reaction temperatures of 42–55°C• Supplied with the recombinant RiboLock RNase Inhibitor• Complete kit includes oligo(dT)_18 and random hexamer primersApplications• First strand cDNA synthesis for RT-PCR and RT-qPCR• Construction of full length cDNA libraries• Antisense RNA synthesis Additional FeaturesThe recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with the reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The kit is supplied with both oligo(dT)_18 and random hexamer primers. The oligo(dT)_18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A), therefore, they can be used for transcription of the 5’-end regions of mRNA or for cDNA synthesis using RNA without the poly(A) tail, e.g., microRNAs. Gene-specific primers may also be used with the kit. The first strand cDNA can be directly used as a template in PCR, real-time PCR, or in second strand cDNA synthesis.