Thermo Fisher, K200001, TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent <i>E. coli</i>, 20 reactions

No. of Reactions: 20 Reactions
Vector: pCRII
Unit Size: Each
The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a 15-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
• Fast and convenient: 15-minute, room-temperature ligation
• Efficient: blue/white screening and >80% clones with correct insert
• Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free: eliminates any enzymatic modifications of PCR product
• Streamlined: does not require use of PCR primers that contain restriction sites
• 3'-T overhangs for direct ligation ofTaq-amplified PCR products
• T7 promoter forin vitroRNA transcription and sequencing
• Versatile polylinker with flankingEcoR I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing
• Taqpolymerase has non-template-dependent activity that adds single deoxyadenosine (A) to the 3' ends of PCR products
• Linearized vector supplied in has single 3' deoxythymidine (T) residues
• Allows PCR inserts to ligate efficiently with vectorCloning, PCR Cloning