Thermo Fisher, K204040, TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent <i>E. coli</i>, 40 reactions

No. of Reactions: 40 Reactions
Vector: pCR2.1
Unit Size: Each
The TA Cloning™ Kit uses the pCR™2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
• Fast & Convenient—15-minute, room-temperature ligation
• Efficient—Blue/white screening and >80% clones with correct insert
• Flexible—Choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free—Eliminates any enzymatic modifications of the PCR product
• Streamlined—Does not require the use of PCR primers that contain restriction sitesThe pCR™2.1 vector provides:
• 3′-T overhangs for direct ligation ofTaq-amplified PCR products
• T7 promoter forin vitroRNA transcription and sequencing
• Versatile polylinker with flankingEcoR I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencingHow TA Cloning™ WorksTaq polymerase has a non-template-dependent activity that adds a single deoxyadenosine (A) to the 3′ ends of PCR products. The linearized vector supplied in this kit has single 3′ deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector. Other Kit ConfigurationsTA Cloning™ Kits are also offered without competent cells (K2020-20, K2020-40), with One Shot™ INVF Chemically Competent E. coli (K2000-01, K2000-40) or with One Shot™ TOP10F Chemically Competent E. coli (K2030-01, K2030-40).