Thermo Fisher, K205040, TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ INVαF' Chemically Competent <i>E. coli</i>, 40 rxns

No. of Reactions: 40 Reactions
Unit Size: Each
The T7 and Sp6 promoters of the pCRII vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCRII cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
• Fast and convenient: 15-minute, room-temperature ligation
• Efficient: blue/white screening and >80% clones with correct insert
• Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free: eliminates any enzymatic modifications of PCR product
• Streamlined: does not require use of PCR primers that contain restriction sites
• 3'-T overhangs for direct ligation ofTaq-amplified PCR products
• T7 and Sp6 promoters forin vitroRNA transcription and sequencing
• Versatile polylinker with flankingEcoR I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing
• How TA Cloning Works\
• Taqpolymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
• Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
• Allows PCR inserts to ligate efficiently with vector
• Kit Configurations
• TA Cloning Kit is offered in a variety of configurations: with One Shot INVF' Chemically CompetentE. coli(K2050-01 and K2050-40), One Shot TOP10F' Chemically CompetentE. coli(K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes Cloning, PCR Cloning