Thermo Fisher, K252002, pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R <i>E. coli</i> and PureLink™ Kit

Quantity: 20 Preps
Unit Size: Each
• pCR™8 TOPO TA vector contains minimal MCS, so sequence more of insert and less of vector
• Patentedattsites make pCR™8 vector is simplest way to create an entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
• pCR™8/GW/TOPO TA Cloning Kit features fast growingE. colithat shortens cloning time
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site
• Spectinomycin resistance gene for robust selection inE. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of insertsCloning,Gateway Cloning,PCR Cloning