Thermo Fisher, K278001, Calcium Phosphate Transfection Kit

Quantity: 75 Reactions
Unit Size: Each
• Based on forming a calcium phosphate-DNA precipitate
• Facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis
• First developed by Graham and van der Ebb and was later modified by Wigle
• Used to transfect a wide variety of cell types for transient expression or for producing stable transformants
• DNA is mixed directly with a concentrated solution of CaCl_2, which is then added dropwise to a phosphate buffer to form a fine precipitate
• Aeration of the phosphate buffer while adding the DNA-CaCl_2solution helps to ensure that the precipitate that forms is as fine as possible, which is important because clumped DNA will not adhere to or enter the cell as efficiently
• Product size: 75 reactionsCell Culture, Plasmid Transfection, Transfection