Thermo Fisher, K4600J10, TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent <i>E. coli</i> cells, 10 Reactions

Name: TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells, 10 Reactions
Brand: Thermo Fisher
SKU: K4600J10

Quantity: 10 Reactions, Unit Size: Each

Catalog Number: K4600J10

Pricing

Send an RFQ.

View RFQ List

Category

Biochemical Test Kits

Pack Size10 reactions

TagsBiochemical Reagents DNA Reagents PCR Cloning Kits

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 Reactions
Unit Size: Each
By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. This kit includes the pCR™II-TOPO TA vector.
• 3'-T overhangs for direct ligation ofTaq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection inE. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficienciesApplications:Cloning, PCR Cloning

Specifications

General

Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Bacterial or Yeast Strain	TOP10
Cell Type	Chemically Competent
Cloning Method	TOPO™-TA
Product Line	TOPO
Product Type	Cloning Kit
Promoter	T7, SP6
Quantity	10 Reactions
Vector	TOPO-TA Vectors
Format	Kit
Unit Size	Each

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.