Thermo Fisher, K494100, BLOCK-iT™ Adenoviral RNAi Expression System

Quantity: 20 reactions
Unit Size: Each
A novel cloning process places an approx. 50-bp DNA oligonucleotide immediately following a U6 pol III promoter into the BLOCK-iT U6 entry vector. After efficient recombination of the entry vector into the pAd/BLOCK-iT-DEST vector, followed by viral production and transduction, the shRNA driven by the U6 promoter can be transiently expressed in most dividing or non-dividing mammalian cell types. The shRNA generated avoids the hosts defense mechanism and will be effective at producing the RNAi gene knockdown response.The pAd/BLOCK-iT-DEST vector offers:
• A promoterless region surrounded byattR sites for efficient recombination with theattL-flanked U6 Gateway entry vector containing the RNAi cassette or anyattL-flanked promoter and gene sequence
• All of the required components for efficient adenoviral packaging and delivery of the shRNA of interest. With this vector, transient analysis of gene knockdown in both dividing and non-dividing mammalian cell types and animal models can be achieved.Adenoviral shRNA, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Virus-Based RNAi