Thermo Fisher, K495510, pLenti6/V5 Directional TOPO™ Cloning Kit

Quantity: 20 Reactions
Unit Size: Each
• Contains TOPO-adapted ViraPower lentiviral expression vector, pLenti6/V5-D-TOPO for quick PCR-based cloning and expression of a target gene in dividing and nondividing mammalian cells
• Vector has CMV promoter for driving high-level, constitutive expression of the target gene and blasticidin selection marker for stable selection in mammalian cells
• High efficiency and rapid cloning
• Produces replication-incompetent virus for enhanced biosafety of system
• Rouse Sarcoma Virus (RSV) enhancer/promoter for Tat-independent production of viral mRNA in the producer cell line
• Modified HIV-1 5' and 3' Long Terminal Repeats (LTR) for viral packaging and reverse transcription
• HIV-1 psi (ψ) packaging sequence for viral packaging
• HIV Rev response element (RRE) for Rev-dependent nuclear export of unspliced viral mRNA
• (CMV) immediate early promoter for high-level constitutive expression of gene of interest in mammalian cells
• C-terminal V5 epitope for detection of recombinant protein
• Blasticidin (bsd) resistance gene for selection inE. coliand mammalian cells
• Ampicillin resistance gene for selection inE. coli
• pUC origin for high-copy replication and maintenance of plasmid inE. coliConstitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery