Thermo Fisher, K601001, Flp-In™ Complete System

Quantity: 1 kit
Unit Size: Each
• Introduce of Flp Recombination Target (FRT) site into genome of mammalian cell line of choice
• Expression vector containing gene of interest is then integrated into genome via Flp recombinase-mediated DNA recombination at FRT site
• Major components include Flp-In. target site vector, pFRT/lacZeo, for generation of host cell line containing integrated FRT site
• Expression plasmid containing a FRT site linked to hygromycin resistance gene for Flp recombinase-mediated integration and selection of stable cell line expressing gene of interest under control of human cytomegalovirus (CMV) immediate-early enhancer/promoter
• Flp recombinase expression plasmid, pOG44, for expression of Flp recombinase under control of human CMV promoter
• Control expression plasmid containing chloramphenicol acetyl transferase (CAT) gene, which when cotransfected with pOG44 into the Flp-In. host cell line, expresses CATBioproduction, Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Stable Cell Line Development, Targeted Integration