Thermo Fisher, L150201, GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing

Name: GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing
Brand: Thermo Fisher
SKU: L150201

Quantity: 1 Kit, Unit Size: Each

Catalog Number: L150201

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Category

Biochemical Test Kits

TagsBiochemical Reagents DNA Reagents PCR Cloning Kits

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 Kit
Unit Size: Each
The GeneRacer Kit provides a method to obtain full-length 5' and 3' ends of cDNA using known cDNA sequence from expressed sequence tags (ESTs), subtracted cDNA, differential display, or library screening. RACE PCR products can be quickly and easily cloned using either the Zero Blunt TOPO PCR Cloning Kit for Sequencing (blunt-end PCR products) or the TOPO TA Cloning for Sequencing Kit (PCR products with 3' A-overhangs). Using the protocols provided, the cDNA ends of rare (30 copies/cell) and long (9 kb) transcripts can be amplified and sequenced starting from 1μg of total RNA.
• Generate cDNA from transcripts at least 10kb in length
• Obtain full-length 5' end of rare transcripts at fewer than 30 copies per cell
• Clone full-length 5' and 3' ends to construct complete cDNA sequenceCloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

Specifications

General

Bacterial or Yeast Strain	TOP10
Cloning Method	TOPO TA
For Use With (Application)	Reverse Transcription
Includes	GeneRacer Module: 2 x 1.5mL Sterile Water, 24μL RNaseOut, 6μL each Calf Intestinal Phosphatase (CIP), CIP Buffer, Tobacco Acid Pyrophosphatase (TAP), 10X TAP Buffer, T4 RNA Ligase, 10X T4 RNA Ligase Buffer, and 10mM ATP, 6 x 250ng GeneRacer RNA Oligo, 2 x 1mL Phenol/Chloroform, 36μL Mussel Glycogen, 200μL Sodium Acetate (3M), 225μL each GeneRacer 5' Primer, 5' Nested Primer, 3' Primer, and 3' Nested Primer, 20μL Control HeLa Total RNA (500ng/μL), 15μL each Control Primer A and Control Primer B.1; SuperScript III RT Module: 6μL SuperScript III Reverse Transcriptase (200U/μL), 24μL 5X First Strand Buffer, 15μL DTT (0.1M), 6μL RNaseH (2U/μL), 6μL Random Primers (100ng/μL), 6μL GeneRacer Oligo dT Primer (900ng/μL), 6μL dNTP Mix (10mM each), 10 S.N.A.P. Columns, TOPO TA Cloning Kit for Sequencing (-20°C), Sufficient reagents and One Shot TOP10 Chemically Competent <i>E. coli</i>
Product Line	GeneRacer, SuperScript, TA Cloning, TOPO
Product Type	Cloning Kit
Quantity	1 Kit
Vector	pCR4-TOPO TA
Format	Kit
Unit Size	Each

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