Thermo Fisher, L32478, Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), fluorescein (FITC)

Quantity: 1 mg
Unit Size: Each
Lycopersicon esculentum (Tomato) Lectin (LEL, TL), fluorescein (FITC), is a bright-green LEL-fluorophore conjugate. The tomato lectin LEL is widely used as marker of blood vessels and microglial cells in rodents and in neuroscience research. It is a valuable tool in studies of rodent tumor angiogenesis and the tracing of neovascular development in xenograft models and brain research. LEL-FITC can be applied directly to tissue sections, or it can be introduced through intravascular perfusion for fluorescence detection.• Very bright FITC conjugate detected with traditional FITC filter set, Ex/Em: 495/515 nm• Applications: immunofluorescence, glycobiology• Sugar specificity: [GlcNAc] 1,3-N-acetylglucosamine• Inhibiting/eluting sugar: chitin hydrolysate• Blood group specificity: non-specific• Unconjugated lectin molecular weight: 71 KDa.• Immunofluorescence working concentration: 5–20 μg/mLLEL is a very stable single-subunit glycoprotein containing ∼50 percent arabinose and galactose and may form multimeric aggregates in solution. It is provided at a 2 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca^2+. If a precipitate forms upon long-term storage, warm to 37°C.