Thermo Fisher, LC5699, HiMark™ Pre-stained Protein Standard

Name: HiMark™ Pre-stained Protein Standard
Brand: Thermo Fisher
SKU: LC5699

Quantity: 250 μL, Unit Size: Each

Catalog Number: LC5699

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Category

Protein Ladders & Markers

Pack Size250 µL

TagsProtein Molecular Weight Markers Gel Electrophoresis Equipment and Supplies Gel Electrophoresis Reagents and Buffers

CollectionsThermo Fisher Gel Electrophoresis: Agarose Gels, SDS-PAGE & Blotting Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 250 μL
Unit Size: Each
• Comprehensive - contains markers with a wide range of high molecular weights, providing nine protein bands in the range of 30 to 460kDa
• Convenient - a ready-to-use formulation eliminates the need to heat, reduce, or add sample buffer prior to use
• Efficient - allows easy visualization of electrophoresis and rapid evaluation of Western transfer efficiency
• Compatible - intended for use with NuPAGE Novex 3 to 8% and 7% Tris-Acetate gels under denaturing conditions
• Can also be used with NuPAGE Novex 4-12% Bis-Tris Gels with NuPAGE MOPS Running Buffer and Novex 4% Tris-Glycine Gel, however to obtain best results with HMW proteins, always use NuPAGE Novex Tris-Acetate Gels
• Gel Compatibility: NovexTris-Glycine Gels, NuPAGE Tris-Acetate GelsHiMark™ High Molecular Weight Protein Standard also available in an unstained format1D Gel Electrophoresis, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

General

Detection Method	Colorimetric
For Use With (Application)	Tris-acetate gels
Gel Compatibility	Novex™ Tris-Glycine Gels, NuPAGE™ Tris-Acetate Gels
Molecular Weight (g/mol)	460, 268, 238, 171, 117, 71, 55, 41, 31 kDa
Quantity	250 μL
Ready to Load	Yes
Recommended Applications	Tris-acetate gels
Shipping Condition	Dry Ice
Number of Markers	9
Product Line	HiMark
Product Type	Protein Ladder
Size Range	30 to 460 kDa
Stain Type	Blue, Pink
System Type	Western Blotting, SDS-PAGE
Unit Size	Each

FAQs

Question: How many reference bands does HiMark Pre-stained Protein Standard contain?

Answer:It contains nine prestained protein bands spanning approximately 31–460 kDa.

Question: Does the protein ladder require heating or addition of sample buffer?

Answer:No. The standard is supplied ready to load directly onto the gel.

Question: Which electrophoresis format is particularly suitable?

Answer:Tris-acetate gel systems are particularly suited to resolving the high-molecular-weight region covered by this ladder.

Application Note

HiMark Pre-stained Protein Standard is a ready-to-load molecular-mass ladder for monitoring high-molecular-weight proteins during SDS-PAGE and assessing protein transfer onto western blot membranes. It contains nine colored reference bands spanning approximately 31–460 kDa, allowing rapid visual inspection of separation and transfer without prestaining the sample proteins. The 250 µL vial is particularly useful for high-mass protein experiments run on Tris-acetate gels, with additional compatibility options depending on gel chemistry. Mix gently and load the appropriate volume for the gel system; heating, chemical reduction and the addition of sample loading buffer are not required for the ladder. Use the marker positions as approximate mobility references rather than absolute proof of a protein's molecular mass. Keep the standard at the specified frozen storage temperature and minimize repeated freeze–thaw cycles.

Ordering

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