Thermo Fisher, M16315, RevertAid™ Master Mix, with DNase I

No. of Reactions: 50 Reactions
Unit Size: Each
For reverse transcription the kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the RevertAid RT Reverse Transcription Kit, while maintaining high performance.Highlights
• Integrated genomic DNA removal step
• Fast—reaction time typically 15–30 min
• Convenient—easy workflow with few pipetting steps
• Reliable—reverse transcription of both abundant and limited targets
• Flexible—optimized two-step protocol, enabling multiple PCR reactions from a single reverse transcription reactionApplications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis