Thermo Fisher, M16325, RevertAid™ Master Mix, with DNase I

Name: RevertAid™ Master Mix, with DNase I
Brand: Thermo Fisher
SKU: M16325

No. of Reactions: 100 Reactions, Unit Size: Each, Thermo Scientific RevertAid Master Mix with DNase I is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit includes recombinant endonuclease (DNase I) to remove contaminating genomic DNA from RNA preps.

Catalog Number: M16325

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Category

PCR Master Mixes

TagsPCR Equipment and Supplies PCR Supplies Reverse Transcription Products cDNA Synthesis Kits and Master Mixes

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

No. of Reactions: 100 Reactions
Unit Size: Each
For reverse transcription the kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the RevertAid RT Reverse Transcription Kit, while maintaining high performance.Highlights
• Integrated genomic DNA removal step
• Fast—reaction time typically 15–30 min
• Convenient—easy workflow with few pipetting steps
• Reliable—reverse transcription of both abundant and limited targets
• Flexible—optimized two-step protocol, enabling multiple PCR reactions from a single reverse transcription reactionApplications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Specifications

General

Final Product Type	First-Strand cDNA
No. of Reactions	100 Reactions
Quantity	100 Reactions
Reaction Format	Premixed Components
Reagent Type	Reverse Transcription
Reverse Transcriptase	RevertAid
Ribonuclease H Activity	Yes
Shipping Condition	Dry Ice
Starting Material	RNA
Technique	Reverse Transcription
For Use With (Application)	RT-PCR
Reaction Speed	30 min.
Unit Size	Each

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