Thermo Fisher, N7991, Newport Green™ DCF Diacetate, cell permeant

Quantity: 1 mg
Unit Size: Each
• Kd for Ca^2+>100μM), making this valuable probe for detecting Zn^2+influx into neurons through voltage- or glutamate-gated channels
• When used alongside dyes with dual Ca^2+/ Zn^2+sensitivity such as fura-2 and mag-fura-2, provides confirmation that changes in Zn^2+levels, and not Ca^2+or Mg^2+are being detected
• The response to transition metals, including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd, has been characterized
• Label (Ex/Em): Newport Green DCF (approximately 505/535nm)
• K_d(Zn^2+) (in buffer): approximately 1μM
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding dissolved indicator to medium containing cellsCell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Zinc and Heavy Metal Ion Detection