Description
Unit Size: Each
Brilliance E.coli/Coliform Selective agar 60mm pk10. Brilliance™ E. coli/coliform Selective Agar (formerly Chromogenic E. coli/coliform Selective Agar) is for the detection and enumeration of Escherichia coli and other coliforms from food and water samples.The recovery and enumeration of Escherichia coli and coliforms are important indicators of environmental and food hygiene. Use Brilliance E. coli / Coliform Selective Agar for detection and enumeration of Escherichia coli and other coliforms in food and water samples.
• Easy-to-read: purpleE. colicolonies are easy to differentiate from pink colonies of other coliforms
• Selective: due to the presence of selective agent sodium lauryl sulphate the medium is selective against Gram-positive organisms.Brilliance E. coli / Coliform Selective Agar contains two chromogenic agents:Rose-Gal: detects ß-galactosidase activityX-Glu: detects ß-glucuronidase activityDetection of ß-glucuronidase activity is widely used to differentiate Escherichia coli, as this is present in E. coli but not in other members of the coliform group. As most coliforms are lactose-positive, ß-galactosidase activity, encoded by the lacZ gene, is then used to differentiate this group from other organisms able to grow on the selective medium. This results in purple E. coli, as they are able to cleave both chromogens with other coliforms, giving pink colonies, as they cleave only the galactoside chromogen.The medium also contains sodium lauryl sulphate which acts as a selective agent, inhibiting the growth of Gram-positive organisms. Due to the addition of tryptophan, the rapid confirmation of E. coli by the formation of indole using a spot indole test is possible.
