Thermo Fisher, PV3176, Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide
Quantity: 800 x 20 μL Assays, Unit Size: Each
Catalog Number: PV3176
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Category
| Assay | Serine/Threonine Kinase Assay |
|---|---|
| Assay Entry | Biochemical phospho-specific activity (no antibody) |
| Excitation/Emission | 405/465, 535 nm |
| For Use With (Application) | FRET |
| Format | 384-well Plate |
| Label or Dye | Methylcoumarin |
| No. of Reactions | 800 x 20 μL Reactions |
| Quantity | 800 x 20 μL Assays |
| Readout | End Point |
| References | BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978) |
| Shipping Condition | Dry Ice |
| Source | Bovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR) |
| Substrate Properties | Peptide-Based Substrate |
| Target Entry | MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E |
| Target Enzyme | Serine/Threonine Kinases |
| Validated Kinases | BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8 |
| Product Line | Z ́-LYTE |
| Unit Size | Each |
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