Thermo Fisher, PV6087, Kinase Tracer 314, 25 μM

Name: Kinase Tracer 314, 25 μM
Brand: Thermo Fisher
SKU: PV6087

Quantity: 25 μL, Unit Size: Each

Catalog Number: PV6087

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Category

Cell Sorters

Pack Size25 µL

TagsCell Analysis Products Cell Based Assays General Cell Based Assays

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 25 μL
Unit Size: Each
• Excitation/Emission (nm): 340/615, 665
• Label or Dye: Alexa Fluor™ 647
• Target Enzyme: Kinases (Multiple)
• Validated Kinases: PIK3CD, PIK3CG, FRAP1 (mTOR)
• In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 314 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

Specifications

General

Assay	Kinase Binding Assay
Assay Entry	Biochemical binding/kinetic
Excitation/Emission	340/615, 665
For Use With (Application)	TR-FRET
For Use With (Equipment)	Microplate Reader
Format	384-well Plate
Label or Dye	Alexa Fluor™ 647
Quantity	25 μL
Readout	Kinetic
Shipping Condition	Dry Ice
Target Entry	PIK3CG, PIK3CD/PIK3R1, mTOR, FRAP1
Validated Kinases	PIK3CD, PIK3CG, FRAP1 (mTOR)
Type	Kinase Tracer
Unit Size	Each

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Thermo Fisher, PV6087, Kinase Tracer 314, 25 μM

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General

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