Thermo Fisher, PV6121, Kinase Tracer 222, 50 μM

Quantity: 25 μL
Unit Size: Each
The LanthaScreen™ Eu Kinase Binding Assay• Is easily optimized• Measures inhibitor binding• Performs simple off-rate measurementsEasily Screen for Kinase BindingThe LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (TR-FRET), while displacement of the tracer with a kinase inhibitor results in a loss of TR-FRET.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.