Description
Unit Size: Each
Use McClung-Toabe Agar for selectively isolate and differentiate Clostridium spp.
• Selective– Neomycin inhibits many gram-positive and gram-negative organisms
• Easy-to-interpret– Based on lecithinase and lipase production byClostridiumspp^1
• Ready-to-use– Convenience of prepared mediaClostridium spp. produce the enzymes lecithinase and lipase. Egg yolk suspension (50%) present in the medium serves as the substrate to detect lecithinase and lipase activity^3. Lecithinase degrades lecithin and produces an insoluble, opaque precipitate in the medium surrounding growth^4. Lipase breaks down free fats in the egg yolk forming in an iridescent sheen on the colony surface.This medium contains gelatin peptone that supplies amino acids and other nitrogenous compounds necessary for the growth of anaerobic bacteria. Dextrose serves as an energy source. Egg yolk serves as a substrate for detection of lecithinase and lipase activity^3 and also reduces the toxic effect of organic peroxides which may accumulate in the medium. Neomycin acts as the selective agent which inhibits many gram-positive and gram-negative organisms. Agar is the solidifying agent.