Description
Quantity: 100/Pk.
Unit Size: Each
Bailey and Lacey modified the medium by substituting Phenol red indicator for Adrade indicator^4. Fermentation reactions are read on the slant and in the butt. Phenol red is an indicator of carbohydrate fermentation whereas, Ferric ammonium citrate is an indicator of H_2S production.
• Ready to use– Solid media
• Recommended for determination of H_2S production by enteric gram-negative bacilli^2and for detection of H_2S produced by some strains ofPseudomonas^3
• Easy to interpretCarbohydrate FermentationPositive test for Slant/Butt Reaction– Yellow (acid)Negative test for Slant/Butt Reaction– Red (alkaline)KIA Color ReactionsRed slat/yellow butt– dextrose (+), lactose (-)Yellow slant/yellow butt– dextrose (+), lactose (+)Red slant/red butt– dextrose (-), lactose (-)Hydrogen Sulphide ProductionPositive test– Black color throughout medium, a black ring at the juncture of the slant and butt, or a black precipitate in the buttNegative test– No black color developmentGas ProductionPositive test– Bubbles in the medium, cracking and displacement of medium, from side and bottom of tubeNegative test– No bubbles and no separation or displacement of the mediumThis medium contains casein and meat peptones that supply nitrogenous compounds, amino acids and vitamins required for bacterial growth. Sodium chloride provides essential electrolytes and maintains osmotic equilibrium. The carbohydrate sources present in the medium are lactose and dextrose. Phenol red is an indicator of carbohydrate fermentation whereas ferric ammonium citrate is an indicator of H_2S production.
